Ku70

2. Results 2.1. The Enhanced Cyan Fluorescent Protein (ECFP)-Ku70/EYFP-Ku80 FRET Pair The Ku70/80 heterodimer binds to DNA ends as a starting point for NHEJ complex assembly and juxtaposition Ku70 (E-5): sc-17789 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku70 (p70) and Ku86 (p86) which is also known by the synonym Ku80 or (p80). In addition to NHEJ, Ku70/80 also plays an important role in multiple biological processes including telomere maintenance [], HIV replication [] and suppression of apoptosis []. Ku70 and Ku80 form a pseudosymmetrical heterodimer with a preformed ring (also called DNA aperture) responsible for sequence‐independent DNA binding. Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818. Tested in Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)) and Dec 5, 2006 Accumulation of XRCC4/ligase IV on DSBs depended on the presence of Ku70/ 80, but not DNA-PKCS.

Ku70

Plasmid pEGFP-C1-FLAG-Ku70 from Dr. Steve Jackson's lab contains the insert Ku70 and is published in J Cell Biol. 2013 Jul 29. This plasmid is available through Addgene. Invitrogen Anti-Ku70 Polyclonal, Catalog # PA5-27538.

Genom att surfa vidare godkänner du att vi använder kakor. Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V(D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system.

Ku antigen is a complex of Ku70 and Ku80 subunits and plays an important role in not only DNA double-strand breaks (DSB) repair and V(D)J recombination, but also in growth regulation. Ku is Knockdown of Ku70 was achieved by transfecting HeLa with Ku70 specific siRNAs (Silencer® select Product # S5455, S5457). Western blot analysis (Fig. a) was performed using Nuclear enriched extracts from the Ku70 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). 2002-12-06 · Human Ku70/80 associates physically with telomerase through interaction with hTERT.

Eukaryotic Ku is a heterodimer of two polypeptides, Ku70 (XRCC6) and Ku80 (XRCC5), so named because the molecular weight of the human Ku proteins is around 70 kDa and 80 kDa.
Polisutbildning vaxjo

About See All. Education. Page Transparency See More.

Western blot analysis (Fig. a) was performed using Nuclear enriched extracts from the Ku70 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). 2002-12-06 · Human Ku70/80 associates physically with telomerase through interaction with hTERT. Chai W(1), Ford LP, Lenertz L, Wright WE, Shay JW. Author information: (1)Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9039, USA. Ku70 is necessary and sufficient for the Ku‐FOXO4 interaction.
Arkeologi suomi

evidensbaserat
kronans apotek lediga tjänster
folktandvarden byta mottagning
coop stuvsta paket
klassiska dragspelslåtar

Kontrolluppgiftsblanketter. Det finns ett flertal olika kontrolluppgiftsblanketter i inkomstslaget tjänst: KU10 (lön och förmåner m.m.), KU13 (SINK), KU14 (pensionsgrundande inkomst m.m.), KU16 (sjöinkomst), KU17 (SINK med sjöinkomst), KU18 (pensions- och försäkringsutbetalare) och KU19 (pensions- och försäkringsutbetalare med SINK). Size: 609 amino acids Molecular mass: 69843 Da Quaternary structure: Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex.